rr036a cell cycle checkpoint antibody sampler kit cell signaling technology  (Cell Signaling Technology Inc)

Journal: Bioengineered

Article Title: Knockdown of mitochondrial threonyl-tRNA synthetase 2 inhibits lung adenocarcinoma cell proliferation and induces apoptosis

doi: 10.1080/21655979.2022.2037368

Figure Lengend Snippet: TARS2 knockdown inhibits cell cycle progression (a) Downregulation of TARS2 inhibits the transition from G1 phase to S phase, as evidenced by a flow cytometric assay. (b) The protein levels of p-RB (ser807/811), CDK2, cyclin E1 and cyclin E2 were decreased and that of p21 waf1/cip1 was increased in TARS2-shRNA transfected LUAD cells. GAPDH is utilized as a reference protein.

Article Snippet: The following antibodies were used in this study: TARS2 (15,067-1-AP, Proteintech, China); GAPDH (60,004-1-Ig, Proteintech, China); Phospho-RB (ser807/811) (9917, Cell Signaling Technology); Cyclin E1(20,808, Cell Signaling Technology); Cyclin E2 (4132, Cell Signaling Technology); CDK2 (18,048, Cell Signaling Technology); p21 Waf1/Cip1 (9932, Cell Signaling Technology); MT-ND6 (A17991, ABclonal, China); CYTB (55,090-1-AP, Proteintech, China); MT-CO1(62,101; Cell Signaling Technology); MT-CO3 (55,082-1-AP, Proteintech, China); MT-ATP8 (26,723-1-AP, Proteintech, China); TOM20 (11,802-1-AP, Proteintech, China); cleaved caspase-3 and cleaved caspase-8 (92,570, Cell Signaling Technology); cleaved caspase-9 (9930, Cell Signaling Technology) [ ].

Techniques: Knockdown, Flow Cytometry, shRNA, Transfection

Journal: Bioengineered

Article Title: Knockdown of mitochondrial threonyl-tRNA synthetase 2 inhibits lung adenocarcinoma cell proliferation and induces apoptosis

doi: 10.1080/21655979.2022.2037368

Figure Lengend Snippet: Knockdown of TARS2 inhibits LUAD cells growth in vivo. (a) Tumor growth curves of mice inoculated with A549 cells infected with TARS2 lentivirus were measured weekly with vernier calipers. (b) Tumor weights at 6 weeks post injection. (c)Protein expression in tumor tissue sections detected by immunohistochemical staining. a – b, p-RB(Ser807/811) expression was decreased in shTARS2-1 group compared with SCRAMBLE group; c – d p21 expression was increased in shTARS2-1 group compared with SCRAMBLE group; e-f, cleaved-caspase 9 expression was increased in shTARS2-1 group compare with SCRAMBLE group; g-h, cleaved-caspase 8 expression was increased in shTARS2-1 group compare with SCRAMBLE group; i-j, cleaved-caspase 3 expression was increased in shTARS2-1 group compare with SCRAMBLE group. The scale bar represents 200 μm.

Article Snippet: The following antibodies were used in this study: TARS2 (15,067-1-AP, Proteintech, China); GAPDH (60,004-1-Ig, Proteintech, China); Phospho-RB (ser807/811) (9917, Cell Signaling Technology); Cyclin E1(20,808, Cell Signaling Technology); Cyclin E2 (4132, Cell Signaling Technology); CDK2 (18,048, Cell Signaling Technology); p21 Waf1/Cip1 (9932, Cell Signaling Technology); MT-ND6 (A17991, ABclonal, China); CYTB (55,090-1-AP, Proteintech, China); MT-CO1(62,101; Cell Signaling Technology); MT-CO3 (55,082-1-AP, Proteintech, China); MT-ATP8 (26,723-1-AP, Proteintech, China); TOM20 (11,802-1-AP, Proteintech, China); cleaved caspase-3 and cleaved caspase-8 (92,570, Cell Signaling Technology); cleaved caspase-9 (9930, Cell Signaling Technology) [ ].

Techniques: Knockdown, In Vivo, Infection, Injection, Expressing, Immunohistochemical staining, Staining